Your work will be assessed during or at the end of each module through a variety of means. Biol.Learning and assessment How you will learn These lung progenitor cells can be differentiated from pluripotent stem cells in vitro. 3 During lung development, lung progenitor cells are generated in lung buds and can differentiate into various functional epithelial cells of the lung.

This disease model can be generated by addition of TGF-α after day 28 of in vitro differentiation. (Right) Ménétrier's disease model with massive gastric folds. 2 A stomach disease model using in vitro differentiation method. Stomach organoid with gastric glands and mesenchyme can be differentiated from ES cells in vitro. (Right) Immunofluorescent staining of stomach organoid with Epcam antibody (red), Desmin antibody (green), and DAPI (blue) for epidermis, mesenchyme, and nuclei, respectively. (Left) HE staining of the differentiated stomach organoid (day 56). 1 Stomach tissue differentiated from mouse ES cells in vitro by 3D culture method. We are also studying novel differentiation methods for these respiratory tissues.įig. Recently, differentiation methods for these lung tissues have been investigated in the scientific community. These primordia proliferate, morphologically divide into multiple branches with the mesenchymal layer, and further differentiate into several kinds of epithelial cells to fulfill respiratory functions ( Fig.3). The lung emerges as lung buds from early gastric tube during development.

Differentiation of lung tissue and tissue regeneration We are currently investigating the mechanisms of gastrointestinal development, and studying these mechanisms using our in vitro model.

We think that this method could be a powerful tool to study the mechanisms of stomach development as well as serve as a unique model for various diseases such as gastric cancer ( Fig.2). Recently, we developed an in vitro differentiation method of mouse ES cells to whole stomach tissue ( Fig.1).

During early development, a primitive gastric tube developed from early endoderm is converted to stomach primordium, and further matures to fundus and antrum tissues covered with gastric glands. Major Research Topics Generation of gastric tissues and their disease modelsĪlthough the stomach is a major organ in our body, the mechanisms of development of this organ are not well known. Moreover, we plan to develop in vitro disease models of these organs and technologies for regenerative medicine in the near future. Our goal is to understand the mechanisms of stomach and lung development to perform in vitro differentiation of pluripotent stem cells into these tissue cells. Understanding of this process could contribute to the development of in vitro differentiation methods. During mammalian development, cells differentiate to form precise 3D structures of organs. Therefore, these stem cells find potential application in regenerative medicine and drug discovery, and it is very important to strictly regulate this potent differentiation ability to induce multi-step differentiation of these stem cells toward functional tissue cells. Pluripotent stem cells, such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, have abilities of unlimited self-renewal and multiple differentiations into all the tissue cells of the body. Professor KURISAKI Akira Assistant Professor TAKADA Hitomi, INTO Atsushi Labs HP Outline of Research and Education

Stem cell technologies